2.5 qPCR assay for estimation of viral load
Amplicons of HAdV-2, hexon gene (nt 18856–19137) were used for the preparation of the standard positive control using primers, probes, and methodology as described earlier (Heim et.al 2003, Koul et.al 2018, Potdar et.al 2020). A standard curve generated by testing ten-fold serial dilutions of standard positive control and plotting the log dilution of standard DNA against Ct value to determine the sensitivity of the assays. The qPCR assay was performed on an ABI 7500 machine (Applied Biosystems Inc, USA).