2.5 qPCR assay for estimation of viral load
Amplicons of HAdV-2, hexon gene (nt 18856–19137) were used for the
preparation of the standard positive control using primers, probes, and
methodology as described earlier (Heim et.al 2003, Koul et.al 2018,
Potdar et.al 2020). A standard curve generated by testing ten-fold
serial dilutions of standard positive control and plotting the log
dilution of standard DNA against Ct value to determine the sensitivity
of the assays. The qPCR assay was performed on an ABI 7500 machine
(Applied Biosystems Inc, USA).