Influence of specific ECM proteins on liver-specific functions
After confirming successful covalent ECM tethering to MPS, we moved to assess biological responses in spheroids by combining hepatocytes and stellate cells with modified MPs (Figure 5A) , specifically to study potential impact of different ECM proteins on liver-specific functions. In culture, viable hepatocytes synthesize and excrete albumin and urea into the surrounding medium, replicating important functions they perform in vivo in the liver. To characterize these critical cellular functions, we collected culture medium and measured albumin and urea production at specific time points.