Figure 4: EDC/NHS coupling reaction for bioconjugation of ECM
proteins to MPs. A) Schematic of EDC/NHS reaction between protein
carboxyl residues and free amine groups from PFC- oxygenating MPs in
varied pHs. B) Quantification of covalently tethered protein (laminin
111) via BCA assay kit. Data represented as mean ± SD, n = 4.
Statistical analysis via one-way ANOVA with Tukey’s post hoctesting with A as the highest mean and C with the lowest mean. C) Atomic
composition of modified and non-modified PFC- MPs measured via XPS. The
N1S percentage correlates to the concentration of proteins attached to
the surface.