The PPLP2-GFP-BirA parasite line was generated by using vector
pARL-pffnpa -GFP-BirA (). The full-length gene was amplified via
PCR using the respective primers (for primer sequences, see Table S4).
Ligation of insert and vector backbone was mediated by KpnI and AvrII
restriction sites. Transfection of the construct was carried out as
described above. Episomal presence of the construct was checked by using
the following primers: PPLP2-BirA-KpnI-FP and pGREP RP (for primer
sequences, see Table S4).