Figure 4. Comparison of results published by Frantzen and Holo
(2019) to those generated by vAMPirus for the lactococcal phage amplicon
sequence dataset. Patterns of lactococcal phage OTU relative abundance
reported by Frantzen and Holo (2019; panel I) were recapitulated in
vAMPirus ncASV results (panel II). Panel I displays original relative
abundances of de novo OTUs based on 99.5% nucleotide similarity
from Frantzen and Holo (2019). Panel II displays vAMPirus-generated
99.5% similarity ncASV relative abundance results. Panels III-VI
demonstrate additional analyses output by vAMPirus, including ASV
relative abundances, aminotype relative abundances, aminotype phylogeny
with nodes colored by sequence phylogroup assignment, and aminotype
phylogroup relative abundances. X-axis labels for all relative abundance
graphs refer to dairy sample identity listed in Table 2. Colors in
panels I-IV are plot-specific; similar colors across these panels donot indicate same sequences/clusters. However, colors assigned to
phylogroups are consistent across panels V and VI. Figure from Frantzen
and Holo (2019) reprinted with permission from authors.
Table 2. Comparison of Lactococcus phage sequence richness per
sample between Frantzen and Holo (2019) results and vAMPirus (this
study) results.