Figure 4. Comparison of results published by Frantzen and Holo (2019) to those generated by vAMPirus for the lactococcal phage amplicon sequence dataset. Patterns of lactococcal phage OTU relative abundance reported by Frantzen and Holo (2019; panel I) were recapitulated in vAMPirus ncASV results (panel II). Panel I displays original relative abundances of de novo OTUs based on 99.5% nucleotide similarity from Frantzen and Holo (2019). Panel II displays vAMPirus-generated 99.5% similarity ncASV relative abundance results. Panels III-VI demonstrate additional analyses output by vAMPirus, including ASV relative abundances, aminotype relative abundances, aminotype phylogeny with nodes colored by sequence phylogroup assignment, and aminotype phylogroup relative abundances. X-axis labels for all relative abundance graphs refer to dairy sample identity listed in Table 2. Colors in panels I-IV are plot-specific; similar colors across these panels donot indicate same sequences/clusters. However, colors assigned to phylogroups are consistent across panels V and VI. Figure from Frantzen and Holo (2019) reprinted with permission from authors.
Table 2. Comparison of Lactococcus phage sequence richness per sample between Frantzen and Holo (2019) results and vAMPirus (this study) results.