Statistical analysis
Medians were used as measures of central tendency. Comparisons between two groups were performed using the Mann–Whitney U test for non-normally distributed variables. The comparison between groups was evaluated using the Kruskal–Wallis test. Correlation analysis was performed using pointwise point biserial correlation analysis. All statistical analyses were performed using GraphPad Prism version 8.0 for Windows (GraphPad Software, Inc., San Diego, CA, USA).