Antibody and reagents
Rabbit anti-Poly/Mono-ADP-ribose (E6F6A) (CST, 83732S) (1:1000), rabbit anti-Flag (ZENBIO, R24091) (1:2000), mouse anti-Flag (ZENBIO, 250111) (1:2000), Rabbit anti-HA (ZENBIO, 390001) (1:2000), Mouse anti-Myc (ZENBIO, 250112) (1:2000), Mouse anti-GFP (EASYBIO, BE2001-100UL) (1:2000), Mouse anti-β -actin (CST, 4970S) (1:2000), Mouse anti-ISG15 (Santa Cruz, sc-166755), HRP-linked horse anti-mouse IgG (CST, 7076S) (1:2000), HRP-linked goat anti-rabbit IgG (CST,7074S)(1:2000), IFN-β (Sino Biological, 10704-HNAS) were purchased from the corresponding manufactures.