Antibody and reagents
Rabbit anti-Poly/Mono-ADP-ribose (E6F6A) (CST, 83732S) (1:1000), rabbit
anti-Flag (ZENBIO, R24091) (1:2000), mouse anti-Flag (ZENBIO, 250111)
(1:2000), Rabbit anti-HA (ZENBIO, 390001) (1:2000), Mouse anti-Myc
(ZENBIO, 250112) (1:2000), Mouse anti-GFP (EASYBIO, BE2001-100UL)
(1:2000), Mouse anti-β -actin (CST, 4970S) (1:2000), Mouse
anti-ISG15 (Santa Cruz, sc-166755), HRP-linked horse anti-mouse IgG
(CST, 7076S) (1:2000), HRP-linked goat anti-rabbit IgG
(CST,7074S)(1:2000), IFN-β (Sino Biological, 10704-HNAS) were
purchased from the corresponding manufactures.