Cell culture and transfection
293T cells were cultured in DMEM (Gibco, 11965092) supplemented with
10% FBS (TransSerum, FS101-02) at 37 ℃ incubator with 5%
CO2. 293T cells were kept in our laboratory.
Polyethylenimine (PEI) (Sigma-Aldrich, 9002-98-6) were used to
transiently transfect DNA constructs into 293T cells.