2.6. Total expressible fluid (TEF) determination
The total expressible fluid (TEF) was determined according to the procedures described by Ismail et al. (2021) and Colmenero et al. (1995) with minor modification. Three replicates of puree (~10 g) at room temperature were centrifuged (1 min, 3250 g), heated in a water bath (30 min, 70 °C) and immediately recentrifuged. In case of microwave oven heating method, three replicates of puree (~10 g) at room temperature were centrifuged (1 min, 3250 g), heated by the household microwave oven (Model DMW799BL, Danby, ON, CA) for 1 min at 700W and immediately recentrifuged. The supernatant was removed and the residue weighed. The total expressible fluid (TEF) was calculated according to the following equation (Ismail et al., 2021):
TEF(%) =\(\frac{Weight\ of\ the\ sample\ before\ heating-\ Weight\ of\ the\ sample\ after\ heating}{\text{Weight\ of\ the\ sample\ before\ heating}}\)× 100 (1)