3.1. Gene expression of OCTN transporters in BeWo cells upon effect of inflammatory stimulants
To determine the effect of inflammatory environment on the expression of genes encoding OCTN1 and OCTN2 (SLC22A4 and SLC22A5 , respectively) in one of the commonly used placental cell line, the choriocarcinoma BeWo cells have been exposed to several cytokines and inflammatory stimulants for 24, 48 and 72 h (Fig. 1 A, B). In the Fig. 1 A, representing the SLC22A4 transporter, we observed that, IFN-γ was able to increase the expression of SLC22A4 after 48 h exposition. Moreover, the TNF-α was able to decrease the expression ofSLC22A5 transporter after 24 and 72 h (Fig. 1 B). A few small changes could be observed in the other inflammation cytokines or stimulators, nevertheless, they have not reached statistical significance.