2.3. Chemicals and Reagents
Cytokines IFN-γ, IL-6 and TNF-α, as well as HMGB1 and LPS were purchased
from Sigma–Aldrich (St. Louis, Missouri, USA). RPMI, fetal bovine
serum, HEPES and phosphate buffered saline (PBS) were bought from
Sigma–Aldrich (St. Louis, Missouri, USA).