2.3. Chemicals and Reagents
Cytokines IFN-γ, IL-6 and TNF-α, as well as HMGB1 and LPS were purchased from Sigma–Aldrich (St. Louis, Missouri, USA). RPMI, fetal bovine serum, HEPES and phosphate buffered saline (PBS) were bought from Sigma–Aldrich (St. Louis, Missouri, USA).