Chromosome level assembly of Hi-C data
We generated 171.54 Gb of raw Hi-C data, consisting of 1,148,659,116
paired-end reads (Table S8 ). After quality control, 169.83 Gb
of clean data remained, containing 99.00% clean paired-end reads, which
were used as input for the BOWTIE2 and LACHESIS Hi-C analysis pipelines.
Finally, 160 scaffolds (representing 97.78% of the total genome length)
were anchored to the seven chromosomes of T. polyphylla with a
length of 403.10 Mb (Fig. 1; Table 1 ). The lengths of the
chromosomes ranged from 44.08 Mb to 79.84 Mb with a scaffold N50 of
57.23 Mb (Table S9 ).