Statistics
Statistical outliers were identified in normal Gaussian data sets using
the extreme studentized deviate (ESD) method, and outliers were
uniformly omitted prior to analysis. Statistical analysis was carried
out using JMP software (SAS), analyzed using linear mixed effects models
with a fixed effect of experimental group and a random effect of
experiment day. Model assumptions of normality and homogeneous variance
were assessed by a visual analysis of the raw data and the model
residuals. Right-skewed data were log or square root transformed.
Experimental group was considered statistically significant if the fixed
effect F test p- value was less than or equal to 0.05. Post-hoc
pairwise comparisons between experimental groups were made using Tukey’s
HSD multiple-comparison test. Graphs of results were shown as mean ± SEM
of untransformed data using Prism version 9 (GraphPad).