Statistics
Statistical outliers were identified in normal Gaussian data sets using the extreme studentized deviate (ESD) method, and outliers were uniformly omitted prior to analysis. Statistical analysis was carried out using JMP software (SAS), analyzed using linear mixed effects models with a fixed effect of experimental group and a random effect of experiment day. Model assumptions of normality and homogeneous variance were assessed by a visual analysis of the raw data and the model residuals. Right-skewed data were log or square root transformed. Experimental group was considered statistically significant if the fixed effect F test p- value was less than or equal to 0.05. Post-hoc pairwise comparisons between experimental groups were made using Tukey’s HSD multiple-comparison test. Graphs of results were shown as mean ± SEM of untransformed data using Prism version 9 (GraphPad).