Experimental Mice and in vivo Treatments
Female AKR/J and C57BL/6 mice at 8-10 weeks old were purchased from The Jackson Laboratory. The animals were housed in specific pathogen free conditions at the Cornell University College of Veterinary Medicine Baker Institute for Animal Health or the University of Washington South Lake Union 3.1 animal facility. Animals were handled according to protocols approved by the Cornell University and University of Washington Institutional Animal Care and Use Committees. For IFN-γ neutralization experiments, AKR/J mice were treated with 1 mg of anti-IFN-γ (XMG1.2) or rat IgG1 (Isotype control) at Day -1, +3, +7, and +11. Day 0 served as the day of infection with 200 T. muris eggs.