Isolation of CD8+ T cells with magnetic beads kits
The CD8+ T cells from PBMCs were isolated by magnetic streptavidin nanobeads of Biolegend MojoSortTMIsolation Kits. Incubating PBMCs with biotin antibody cocktail followed by incubation with magnetic streptavidin nanobeads. Targeted cells were depleted by magnetically labeled fraction using a magnetic separator, while untouched CD3+CD8+ cells were collected.