K562 cell lines transfected with HLA-E molecule
An immortalized chronic myelogenous leukemia cell line K562 with MHC
molecules expression deficiency was obtained from ATCC (CCL-243). We
stably transfected K562 cell lines with single allele HLA-E*0103 or
HLA-E*0101 to generate mono-allelic cell lines with lentivirus
transfected methods. Stably transfected cells showed strong expression
(>95%) of HLA-E*0103 or HLA-E*0101 according to flow
cytometry (FCM) analysis.