2.1. Materials
In this study, we used JWH-018
(CAS No: 209414-07-3; Lipomed, Switzerland) compound with molecular
formula C24H23NO. It was stored at +4°C
until JWH-018 was used. JWH-018 and its metabolites were acquired from
Lipomed AG Switzerland. Ketamine hydrochloride (Ketasol %10;
richterpharma ag, Wels, Australia) and xylazine (Xylazinbio %2; Bioveta
Ivanovice na Hané, Czech Republic) were purchased from Biotek Animal
Health Products, Malatya, Turkey. Primary rabbit‐polyclonal caspase‐3
antibody (Neomarker; Lab Vision Corp., Thermo Scientific, Fremont, CA,
Cambridge, UK) and mouse monoclonal desmin antibody (Santa Cruz
Biotechnology, CA, USA) were used.