Figure 3. a.Normal heart histology. H&E x100, b. Wavy appearance in
myocytes and increased eosinophilia in SALD and SAHD groups (arrowhead).
H&E x100, c. In the SALD and SAHD groups, areas consistent
with the findings of ischemia accompanied by karyolysis (arrow), which
are more clearly seen in the lower right and upper left part of the
figure. H&E x100.
Desmin expression in groups was
demonstrated in brown with 3,3’-Diaminobenzidine (DAB) chromogen by
using immunohistochemistry method. When the severity and prevalence of
staining with desmin antibody in the groups were evaluated; Weak,
moderate and intense staining patterns were observed. It was noted that
the control group showed a moderate and homogeneous staining pattern in
terms of staining intensity and prevalence. This pattern was consistent
with the staining pattern seen in myocytes in normal heart tissue. In
SALD and SAHD group cases in which contraction bands were prominent,
desmin accumulation was observed to be intense, especially in myocyte
firming aggregates. In the cases of these two groups, it was noted that
desmin stained weakly in terms of staining intensity and intensity in
the ischemia areas seen as small foci (Figure 4b and 4c). Staining of
SALD and SAHD group cases with desmin antibody was consistent with
histomorphologically observed early signs of acute myocardial infraction
and ischemia findings.