3.6. LC-MS/MS Analysis
In the quantification of JWH-018 and its metabolites by LC-MS/MS
analysis, JWH-018 and JWH-018 metabolites could not be detected in the
control and ALD groups in the heart tissue. JWH-018 and all other
JWH-018 metabolites except for JWH-018 N-(2-hydroxypentyl) derivative
were determined in the AHD group. Levels of JWH-018 and metabolites were
found as follows: JWH-018 (6.868±5.117) ng/mg, JWH-018
N-(3-hydroxypentyl) (0.103±0.048) ng/mg, JWH-018 N-(4-hydroxypentyl) and
N-(5-hydroxypentyl) (0.129±0.069) ng/mg, JWH-018 N-pentanoic acid)
(0.148±0.038) ng/mg respectively . JWH-018 was determined in SA
JWH-018 groups, SALD (0.068±0.0499) ng/mg, SAHD (0.626±0.418) ng/mg
whereas JWH-018 metabolites were not detected all results were given in
the Table 5.
Table 5. The number and
amount of JWH-018 and JWH-018 metabolites determined in the heart
tissue.