Figure 4. a. Normal staining in myocytes with desmin antibody (Control group, Score 2) x200, b. Slightly decreased staining and moderately increased contraction bands in myocytes with desmin antibody (SAHD group, Score 3) x200, c. Decreased staining and increased contraction bands in myocytes with desmin antibody (SALD group, Score 1) x200.
Tonsillar tissue was used as a positive control in the same glass and it was observed that the caspase-3 antibody worked in this positive control (Figure 5a). Heart tissues in all groups were stained negatively with caspase-3 antibody.