DNA sampling
Between April and May 2017, a total of 142 colonies of R.
flaccida and 135 of C. surinamensis were sampled in twelve local
populations inside the permanent study plots (Table S1), in six small
patches: 1-ha (n = 3) and 10-ha (n = 3); and six large
patches: 100-ha (n = 1) and continuous forests (n = 5).
Each study plot is mapped into twenty-five subplots of 20 × 20 m (400
m2). Within each 400 m2 subplot,
host leaves occupied by at least one colony were collected at regularly
spaced intervals of 10 m minimum distance. Single-species colonies
growing on the leaf were prioritized. In the laboratory using a
dissecting scope, 0.5 – 1 g of the bryophyte gametophyte was carefully
separated from the leaf surface avoiding contamination from host plant
leaf tissue or other epiphyllous organisms. Plant tissue was dried using
silica gel after collection. Colonies sampled varied from eight to
fifteen in each reserve. Specimens detailed information are presented in
Table S2.