DNA extraction, and Genotyping by sequencing
Samples were frozen in liquid nitrogen and total genomic DNA was
extracted using an E.Z.N.A. Plant DNA DS Mini kit (Omega Bio-tek, Inc,
Norcross, GA, USA). A double digest Genotyping by sequencing (GBS)
library with the enzymes Pstl/Mspl (Abed et al., 2019) was prepared and
sequenced on the Ion Proton instrument by the Plateforme d’analyses
génomiques (Institut de Biologie Intégrative et des Systèmes (IBIS),
Laval University, Quebec City, QC, Canada). Each sample was identified
using a unique forward and reverse 13-15 base-pairs barcode at the 5’
and 3’ end to identify each individual when multiplexing.