2.5 Database search and quantitative proteomic analysis
The raw data were processed by Proteome DiscovererTM(version 2.4, Thermo Fisher, Waltham, MA, USA) and then identified by Protein Pilot Software 5.0 (AB SCIEA, USA) in combination with SwissProt (version 2021.06)/UniProt (version 2021.09) databases. The search parameters are as follows: cys alkylation, iodoacetamide; static modification, carbamidomethyl; dynamic modification, oxidation of acetyl; proteolytic enzyme, Trypsin (Full); maximum number of missed cleavage allowed, 2; precursor mass tolerance, 20 ppm; fragment mass tolerance, 0.02 Da; quantitative method, TMT 6Plex. For the quantification of TMT, the ratio of ion intensities reported by TMT in the MS/MS spectra of the original data set was used to calculate the fold change (FC) between samples. FC ≥ 1.2 or ≤ 0.83 and p -value < 0.05 were set as cut-off values to designate significantly differentially expressed proteins.