2.7 Parallel reaction monitoring analysis
Majorbio Bio-pharm Technology Co., Ltd. utilized PRM to validate the
abundance of 17 candidate proteins related to the synthesis of secondary
metabolites (Shanghai, China). For the PRM analysis, three biological
replicates from each group were considered. Based on the TMT data, the
signature peptides of the targeted proteins were identified, and only
distinct peptide sequences were chosen. The chromatographic column,
orbit trap and electrospray voltage, protein identification library, and
raw data processing software used for the LC-MS analysis in this
investigation were identical to those used for the TMT analysis.