3.5 Determination of multiple bioactive constituents
The main bioactive components in TH are reported to be phenolic acids and flavonoids [27,28]. To investigate the potential relationship between metabolite accumulation and protein expression, the content of main active constituents was determined simultaneously by UFLC-QTRAP-MS/MS. The condition of UFLC-QTRAP-MS/MS and method validation studies were reported in a previous study [14]. A total of 16 constituents including 12 flavonoids and 4 phenolic acids were quantitated in TH from two SS and FXS. The contents of major flavonoid glycosides (quercitrin, hyperoside, auicularin, quercetin-3-O -β -D -glucuronide, and rutin), and (+)-Catechin in TH from SS were higher than those in TH from FXS. There was no significant difference between the two groups in the content of other constituents (Figure 5 and Table S8).