2.8. Biological characterization of LOC
The proliferation of hepatocytes in the LOC platform was determined using a cell counting kit (CCK-8, Dojindo, Korea). WST-8 solution was added to the culture medium containing the 3D liver sinusoid model. The medium was then incubated for 2 h at 37°C in a humidified 5% CO2 environment. The absorbance at 450 nm was recorded after 100 µL of the solution was transferred to a 96-well plate.