Conclusion
In this study, we developed a portable, low-cost, and simple glucose
monitoring device using a conductive PLA-based three-electrode
electrochemical sensing system, which can be easily integrated with the
OOC platform. The glucose sensor was fabricated using the FDM method,
and the electrode surface was modified by coating it with Nafion/MWCNT
composites to improve its stability and biocompatibility. To determine
the sensitivity of the glucose biosensing device, we bioprinted a
perfusion-based liver-on-a-chip (LOC) platform. The biological
characterization of the LOC demonstrated the proliferation of
hepatocytes along with the expression of all liver-specific genes.
Finally, a drug-toxicity study of the LOC biosensor device was
performed, which demonstrated its ability to measure a broad range of
glucose concentrations.