3. IP-10 determination
IP-10 concentration was measured in the remaining supernatants from QFT tubes using the Quantikine ELISA Human IP-10 Immunoassay (R&D Systems Europe, Ltd., Abingdon, UK) according to the manufacturer’s instructions. All samples were measured in duplicate. The plasma was diluted 1:10 and samples with values outside the upper limit of the standard curve were diluted and measured again. The lower limit of detection (sensitivity) was calculated to be 2.1pg/ml. IP-10 concentrations are expressed in pg/ml.