Table 2. Comparison of rumen papillae measurements of adult male
deer with different begging behaviour
Figure 1. Western blots showing the abundance of UT-B2 urea
transporter and MCT1 short-chain fatty acid transporter proteins in male
adult deer rumen. (A) Comparison was made between signals obtained for
50 kDa UT-B2 and 43 kDa MCT1 in four animals from the November and
January groups. (B) Densitometric analysis of these signals
showed that there was no significant change for either UT-B2 or MCT1.
Data shown as mean ± standard error. Key: NS = not significant.
Figure 2. Western blots showing the ruminal abundance of UT-B2
urea transporter and MCT1 short-chain fatty acid transporter proteins in
male adult deer with different begging behaviour. (A)Comparison was made between signals obtained for 50 kDa UT-B2 and 43 kDa
MCT1 in three rare beggars (M1-M3) and three consistent beggars(M4-M6) . There were also two samples from other consistent
beggars – one juvenile male (J1) and one adult female(F1) . Coomassie staining for all 8 protein samples is also
shown. (B) Densitometric analysis of these signals showed that
there was no significant change for MCT1, but that UT-B2 abundance was
significantly increased in consistent beggar male adults. Data shown as
mean ± standard error. Key: NS = not significant.
Figure 3. Immunolocalization studies showing the location of
MCT1 and UT-B2 transporters in adult male deer rumen. (A) MCT1
protein (strong brown staining) was localized to the stratum basale
layer (marked by arrowhead) in the M5 consistent beggar male
adult deer. The absence of the primary anti-MCT1 antibody prevented any
such staining (see inset). (B) UT-B2 protein (weak brown
staining) was also localized to the stratum basale layer (again marked
by arrowhead) in the M4 consistent beggar male adult deer. The
absence of the primary hUTBc19 antibody prevented any such staining (see
inset). In each image, the scale bar represents 100µm.
Figure 4. Immunolocalization studies showing the location of
MCT1 transporters in various deer rumen. Moderate or strong stratum
basale staining was observed in the rare acceptor adult males(M1-M3) . Strong or very strong MCT1 staining was observed in
the consistent acceptor adult males (M4-M6) , again in the
stratum basale layers. Finally, similar strong staining was also
observed in the juvenile male (J1) and adult female(F1) rumen. Scale bar represents 50µm.
Figure 5. Immunolocalization studies showing the location of
UT-B2 transporters in various deer rumen. No obvious UT-B2 staining was
observed in the rare acceptor adult males (M1-M3) . In contrast
weak-to-moderate UT-B2 staining was observed in the consistent acceptor
adult males (M4-M6) , always in the stratum basale layer.
Moderate UT-B2 staining was also observed in the stratum basale in the
juvenile male (J1) and adult female (F1) rumen. Scale
bar represents 50µm.