2.2. Antibodies
To investigate the protein abundance and localization of both UT-B2 and
MCT1, various antibodies were utilised. The widely used polyclonal
antibody UT-Bc19 raised in rabbits (Walpole et al., 2014) was used to
detect UT-B2 protein. A commercial polyclonal antibody raised in
chickens against rat MCT1 (AB1286-I, Millipore) was also utilised. The
corresponding HRP-linked secondary antibodies used for this study were
anti-rabbit (656120, Biosciences Ltd.); anti-chicken (A16054,
Bio-sciences Ltd.).