Histopathology
CIA mice were sacrificed, and the joints were removed. The specimens were then fixed in 10% phosphate-buffered formalin and decalcified for 30 days in 10% EDTA or 5% formic acid. After decalcification, the joints were embedded in paraffin and histological examination was performed using hematoxylin and eosin (HE) staining (Servicebio, Wuhan, China).