Enzyme-linked immunosorbent assay
Co-culture supernatant and plasma were collected, and IgG and TNF-𝛂 level were determined by ELISA using the Mouse IgG or TNF-𝛂 ELISA Kit according to the manufacturer’s instructions (Multisciences).
Statistical analysis
GraphPad Prism software (v.8.0, San Diego, CA) was used to analyze. Student’s t-test and one-way analysis of variance were used for comparisons between two groups or multiple groups, respectively. For clinical assessment, two-way ANOVA was used to determine significant differences in arthritis scores. P < 0.05 was considered statistically significant. P ‐values are depicted as follows: *P  < 0.05; **P  < 0.01; ***P  < 0.001.