2.1 Insects
In July 2020, we collected three kapok branches that had withered in the
upper stalk due to G. cantor infestation from Qingxiu Mountain
(22°12′-23°32′N, 107°45′-108°51′E), Guangxi Zhuang Autonomous Region
Nanning, China. The stems were incubated in four bubble containers in
the yarn in a cage (50 cm × 70 cm × 80 cm) and sprayed with water every
5 days in a controlled environment (25 ± 1°C, 75 ± 5% RH, 14 L: 10 D;
Lu et al., 2011). After 2 months, five males and five females were
randomly selected from our caged adults and transferred to separate
glass containers (5 cm diameter x 16 cm length) in a refrigerator at -20
°C. After 25 minutes, the adult insect returned to normal and its
mandibles, labial papules and mandibular papules were cut under a
stereomicroscope PX-1 (Camsonar Technology Co., Ltd., Beijing, China).
The mandibles, labial papules and mandibular papules are prepared.