Growth monitoring in the 96-well plate reader
C ells were diluted in the appropriate growth media to
OD600 = 0.55, and 10 µL of the diluted cells were
transferred into 100 µL of growth medium in a 96-well plate (Anicrin,
Flat bottom, #18EMP.I.). The 96-well plate edges were filled with
distilled water or 1xPBS. The remaining 60 wells were used to monitor
the growth. At least one column was always set as a sterility control.
Alamar Blue reagent (BioRad, #BUF012B) was used per the manufacturer‘s
protocol (excitation 545 nm, emission 590 nm). BioTek Synergy MX or H1
microplate readers were used.