Simulated ILDF System
The simulated ILDF system consists of the same or similar equipment used in the batch process. The equipment was arranged in a manner supporting multiple independent concentration/buffer exchange passes with opportunity for sampling and dilution with buffer in between each pass (Figure 1D). The hollow fiber cartridge was not replaced in between passes. A new hollow fiber was used for each bulk run on the simulated ILDF system.
The starting solutions and liposome mixture as described previously were used as a starting material. The buffer solution was used to pre-dilute the liposome mixture as described in the Results Section.