2.6 GOx Nanoreactors Characterization
The nanoreactor catalytic activity was characterized by a
peroxidase-coupled reaction followed by a colorimetric assay. The last
product in this coupled reaction is tetraguaiacol, which absorbs at 470
nm. The increased absorbance is directly related to the glucose
transformation rate. A Michaelis-Menten regression was performed by
preparing reactions at different glucose concentrations (0, 25, 50, 100,
and 150 mM) to describe the catalytic parameters of the VLP-GOx
nanoreactors.
The encapsulation of the GOx enzyme in VLP nanoparticles and the HSA
coating was confirmed by SDP-PAGE. Finally, the size and morphology of
the nanoreactor were studied with transmission electron microscopy (TEM,
JEOL-2010, JEOL) operated at 100 kV.