Micro-PET/CT studies of the 68Ga-HZ20 probe in normal mice
PET images of normal KM mice showed that the probe entered the body and was rapidly distributed with blood circulation and metabolized by the kidney and bladder (Fig. S2A). The maximum single-voxel standardized uptake value (SUVmax), which is the standard nuclear medicine metric, was chosen to show the probe accumulation in tissues or organs by measuring the maximum voxel value in a volume of interest (organs) standardized to patient mass and administered activity. The SUVmax showed that the probe was rapidly cleared from the mouse heart blood pool from 0.51±0.04 to 0.15±0.04 (p=0.0004) at 60 min after injection, and the remaining probe mainly accumulated in the kidneys at 1.3±0.16 (Fig. S2B). Although the uptake values of the liver (0.43±0.02) and lungs (0.43±0.03) were similar, the density in the liver was much greater than that in the lungs, suggesting that the uptake of probe was higher in lung tissue than in liver tissue.