Micro-PET/CT imaging of mice model
7.4 MBq/200μL 68Ga-HZ20 was injected into model mice through the tail vein, anesthetized with isoflurane (2-3%, 1 L/min oxygen), and placed on a heated bed for Micro-PET/CT imaging scanner to statically acquire PET/CT images for a certain period of time. 50-fold unlabeled HZ20 and 7.4 MBq 68Ga-HZ20 were co-injected into mice for blocking studies. For ex vivo imaging of the target tissue, the weight of the organ was weighed, and Micro-PET/CT imaging is performed directly, and the γ-counter assay was performed immediately following the PET imaging procedure. A region of interest (ROI) was plotted on the CT image and mapped onto the PET to further acquire its SUVmax value for further analysis.
Western blot detection of rhACE2 protein in injection site and immunohistochemical detection of ACE2 at organ background level.[Supplementary Materials]