Micro-PET/CT imaging of mice model
7.4 MBq/200μL 68Ga-HZ20 was injected into model mice
through the tail vein, anesthetized with isoflurane (2-3%, 1 L/min
oxygen), and placed on a heated bed for Micro-PET/CT imaging scanner to
statically acquire PET/CT images for a certain period of time. 50-fold
unlabeled HZ20 and 7.4 MBq 68Ga-HZ20 were co-injected
into mice for blocking studies. For ex vivo imaging of the target
tissue, the weight of the organ was weighed, and Micro-PET/CT imaging is
performed directly, and the γ-counter assay was performed immediately
following the PET imaging procedure. A region of interest (ROI) was
plotted on the CT image and mapped onto the PET to further acquire its
SUVmax value for further analysis.
Western blot detection of rhACE2 protein in injection site and
immunohistochemical detection of ACE2 at organ background level.[Supplementary Materials]