3.1.2 Hydrophilic Interaction Liquid Chromatography
Hydrophilic Interaction Liquid Chromatography (HILIC), which has a complementary effect with reverse chromatography, but is also different from normal phase chromatography, can retain strongly polar hydrophilic compounds. 41 HILIC does not use a more toxic mobile phase. The characteristic of HILIC is that its stationary phase is bonded with various polar functional groups. 42 Mohit et al. 43 used HILIC - UV to achieve identification and quantification of five potential genotoxic impurities, and compared the separation effect of zorbax silica column, ZIC-HILIC column and nitrile-HILIC column. The zorbax silica column can give the most ideal analysis results. Marta et al. 44 developed an alternative direct chiral HPLC method to separate the (R)-and (S)- ramosetron on a chlorinated cellulose-based chiral stationary phase in HILIC mode. A new method provided to analyze the enantiomer of ramosetron.