2.3 | Twitching motility
16 ± 1 h grown R. solanacearum F1C1, and 6 h grown E. coliDH5α cells were serially diluted. 5.0 µl of this 105CFU ml-1 concentration was spotted at the centre of their respective nutrient-rich agar plates and incubated at their respective optimal temperatures of growth. The Petri plates containing the R. solanacearum F1C1 cells were observed for 10:00-22:20 h of spotting while E. coli DH5α cells were observed within 4:00-8:00 h duration of spotting under Life technologies EVOS FL inverted microscope with 40X magnification using the 4/10 PH condenser annulus.