MATERIAL-METHOD
The study used 32 male Sprague Dawley rats weighing between 250-300 g
and aged 4-6 months. The rats were obtained from the İnönü University
Experimental Animals Production and Research Center and were housed in a
temperature (21±2°C) and humidity (60±5%) controlled room with a 12:12
h light: dark cycle. The rats were fed a standard chow pellet diet and
had access to pelleted diet and drinking water ad libitum. The study
protocol was approved by the Ethics Committee on Animal Research under
the Faculty of Medicine at İnönü University, Malatya, Türkiye (protocol
no: 2019/A-21). All experimental procedures were carried out by
investigators that blinded the experimental groups and in accordance
with the ARRIVE guidelines [12].
A simple randomization technique was used to assign animals to the
experimental groups (n=8 for each group):
Control group: A single dose of 2 mL saline was intraperitoneally
(i.p.) administered, and the experiment was terminated 72 hours later.
CIS group: A single dose of 8 mg/kg CIS was i.p. administered,
and the experiment was terminated 72 hours later.
RAN+CIS group: The rats were given 50 mg/kg RAN orally for 5
days, and a single dose of 8 mg/kg CIS was administered i.p. on the 3rd
day. The experiment was terminated on the 6th day.
CIS+RAN group: A single dose of 8 mg/kg CIS was administered i.p.
at baseline, and the rats were given 50 mg/kg RAN orally for 5 days
starting from the 3rd day. The experiment was terminated on the 6th day.
Rat weights were recorded prior to anesthesia. General anesthesia was
induced with 5 mg/kg xylazine and 75 mg/kg ketamine i.p. confirmed with
a finger pinching response. Intracardiac blood samples were collected to
measure biochemical parameters such as blood urea nitrogen (BUN),
creatinine (Cre), albumin, Na+, Cl-,
K+, and Ca2+. The blood was
centrifuged at 3000 rpm for 10 minutes, and the serum was separated for
spectrophotometric measurement of biochemical markers in the Architect
C16000 device (Abbott, Chicago, IL, USA) following the manufacturer’s
instructions. The right kidneys were removed, weighed on a
high-precision laboratory scale, and stored at -80 degrees for analysis
of tissue biochemistry parameters such as superoxide dismutase (SOD),
catalase (CAT), reduced glutathione (GSH), and malondialdehyde (MDA).
The capsule of the left kidney was carefully peeled, washed with saline,
and kept at room temperature in 10% formaldehyde-containing boxes for
histopathological analysis.
CISPLATIN DBL 100 mg/ 100 mI Injectable Solution (ORNA, Istanbul) and
Latixa 375 Mg Extended-Release Tablet (Menarini, Istanbul) were used as
the chemical preparations.