Death and Weight
During the course of the experiment, it was observed that three rats expired within the CIS+RAN group. In order to achieve uniformity in sample size across all groups and to obtain sufficient data for statistical analysis, three additional rats were included in the same group. Upon completion of the experiment, the weights of the rats and their respective kidneys were measured. Notably, no statistically significant differences were observed among the groups (p>0.05) (Figure 1).