Histological Analysis
Following tissue follow-up procedures, 4-5 μm thick sections were obtained from the paraffin blocks. The sections were subjected to hematoxylin-eosin (H&E) staining to examine general morphological structures.
Kidney sections were evaluated for tubular degeneration (cast formation, tubular necrosis, and dilatation) in the cortical and medullary areas. Ten randomly selected areas were assessed based on the degree of histological changes and scored as follows: 0 for no change, 1 for mild, 2 for moderate, and 3 for severe changes [13].
The analyses were performed using the Leica Q Win Image Analysis System (Leica Micros Imaging Solutions Ltd., Cambridge, UK) with the Leica DFC-280 research microscope.