In –vivo (7 day treatment)
Four group having four animals each was taken for this experiment. Group 1 was control, Group 2 was treated per day with 20 mg of DAS, Group 3 was treated with 8 mg of chalcone and Group 4 was treated with a combination of DAS (20mg) + Chalcone (8mg). These animals were treated for 7 days and sacrificed on the 8th day. All animals were kept under UV radiation for 2 hours a day, until sacrificed. . UV radiation was given for the purpose of immune compromisation.