Expression of HIF1α protein
HIF1 expression was high in the DAS-24 hr group and then appeared to gradually decline with time as well as with the second dosage. Yet, in the Chalcone-treated group, HIF1 is observed to gradually express more with time and the second dose. In the DAS+Chalcone group of animal hepatic tissue, the expression of HIF1 was essentially the same. This justifies why DAS+chal have little impact on the HIF1 group (Fig-4a, b). The band density of HIF1 expression is depicted in the bar diagram, which also clearly shows the pattern of HIF1 expression. (Fig-4c).