In vivo experiment
Following a single dosage of DAS on day 1, the hepatic tissue animal
group treated with DAS demonstrated a progressive increase in the
SULT1E1 expression with increasing duration. SULT1E1 expression was
comparable to DAS 48 hours after DAS 2nd dose group.
On the other hand, in 24 and 48 hours groups of chalcone treated animals
expressed highest SULT1E1 but the expression gradually decreased with
increasing duration. The combination group (DAS + Chal) showed an
expression level less than the DAS or Chalcone alone group but greater
than the control group (Fig-4a).