Cytosol preparation of human breast tissue sample
Breast tumor and the corresponding surrounding tissues were homogenized (30% w/v) in ice-cold phosphate buffer (0.1 mol /L, pH 7.4); andcentrifuged at 10,000 rpm at 4ºC for 30min. The supernatant (cytosol) was collected and stored at -20˙C in different aliquots for further assays.