In vitro experiment
The DAS group expressed SULT1E1 more than the control group during the
in-vitro therapy, and this expression gradually increased over time. The
chalcone group had less SUT1E1 expression than the control and gradually
decreased with increasing time. Whereas the combination group had
highest expression at the first 3 hours of incubation, decreased at 5
hours and remain undetected at 12 hours of incubation (Figure-4b).
However, 3hr DAS+Chal treated tissue showed the best result with highest
level of SULT1E1 and then gradually decreased in 5 hr and least is found
in 12 hr. Thus, DAS has direct effect on the expression of SULT1E1 and
Chalcone has some moderate effect.