Glutathione peroxidase (GPx) activity study by gel zymogram
The GPx activity was highest in the control group (Untreated) as compared to other group of animal such as DAS alone, Chalcone alone and DAS + Chalcone group. The GPx activity was significantly lower in the DAS group than it was in the chalcone, which was likewise significantly lower. The DAS + Chalcone group a gradual increase in the GPX activity with duration and the highest activity among them all was found in the DAS + Chalcone 2nd dose group (Fig-3c).
The control group showed some GPx activity and there was no activity in the DAS treated animal group. The Chalcone group showed minute activity. The Das + Chal group showed high Gpx activity. The GPx activity was found to be lower in the DAS, Chalcone group as compared to the control group of animal. The GPx activity was stimulated in the DAS+Chalcone group of animal’s hepatic tissue (Fig-3a).