SOD (24 to 72 hours)
The SOD activity remained unchanged in the DAS alone, chalcone alone group with a slight decrease of activity in DAS group. The combination group showed a decrease in the activity in the DAS+Chalcone (48 hr) and then markedly increased in the 72 hour group and the group treated with the 2nd dose. DAS alone and Chalcone alone group showed comparatively less SOD activity than the control or untreated group. The combination group of DAS+ chalcone showed a significant increase in SOD activity on treatment with the 2nd dose after 72 hours of the 1st dose. Among the 3 control animals, SOD activity was high in 2 animals and less in the 3rd. The DAS treated 24, 48 and 72 hour animal showed Less SOD than first 2 controls. Animal treated with 2nd dose of DAS had higher SOD activity. The chalcone treated group had low SOD activity than control and DAS group, but the 2nd dose had high SOD activity. Interestingly DAS+Chal had the highest SOD activity, which gradually increased with duration till 72 hrs (Fig-3c).