Wound closure assay
In 6 well plates, U2OS WT, MMP-2 KO and GFP-MATK stable cells were
cultured, separately, for 24 hours under standard culture conditions to
reach 100% confluency. Once confluent, a vertical scratch was formed
down the middle of the well using a 200 µl pipette tip. Cells were
washed with 1x PBS and OPTI-MEM was added to each well. The appropriate
concentrations of doxorubicin were added to the OPTI-MEM in respective
wells. Images of the wound were taken at 0 hour, 24 hour and 48 hour
time points. The percent wound closure at 24 and 48 h were calculated as
the following: [(Initial wound width-final wound width)/(initial wound
width) *100].