Cell Culture
Human osteosarcoma cell line (U2OS; ATCC-HTB-96) were cultured at 37℃ in
a humidified 5% CO2 atmosphere in DMEM supplemented
with 10% FBS. U2OS WT+ GFP-MATK stables were additionally supplemented
and cultured with 4 µg/mL puromycin. MMP-2 knockout U2OS cells were
generated by CRISPR/Cas-9, as previously described
(7).