Table 3. Plasmids used in this study
Plasmid Genotype Description Reference/source*
pCV1 araC+ bla+ ParaBAD expression vector (VanDrisse and Escalante-Semerena, 2016)
pTEV19 bla+ Overexpression vector for MBP-H6- N-terminally tagged rTEV-cleavable protease (VanDrisse and Escalante-Semerena, 2016)
pPagR7 pagR+ bla+ pagR+ (stm2345+) cloned into pCV1
pTktC1 tktD+ tktE+ bla+ tktD+ and tktE+ cloned into pCV1
pTktA1 tktA+ bla+ tktA+ cloned into pCV1
pTktB1 tktB+ bla+ tktB+ cloned into pCV1
pPagR-8 pagR+ bla+ pagR + (stm2345+) cloned into pTEV19
pCP20 flp+ bla+ cat+ FLP expression plasmid, temperature sensitive above 37 °C (Cherepanov and Wackernagel, 1995)
pKG136 lacZY+ kan+ For FLP integration of lacZY+ kan+ cassette at FLP-resolved deletion sites J. M. Slauch