1.1 Test strains
The D. flagrans isolate used in this study was F088 (CGMCC No.
11209), the gene database entry number is KU881774.1, which was isolated
and purified from sheep feces collected in Heilongjiang Province, China
in 2014 by an improved spreading separation technique. The isolate was
stored in refrigerator at 4 ℃ on test tube containing 2% cornmeal agar.
Mycelium and spores were taken from the preserved inclined plane and
inoculated on Potato Dextrose Agar (PDA, Beijing Landbridge Technology
Co., LTD.) plate containing chloramphenicol and incubated at 28 ℃ for 7
days.