2.1 Determination of cAMP content in spores
The standard cAMP concentration (0.3125–0 pmol/50 μL) and its
corresponding absorbance were linearly correlated. Based on the measured
data, the standard curve is shown in Figure 1, and the following
regression equation is obtained: Y = −0.2214X + 0.1949. In the formula,
Y represents the measured absorbance value, X represents the standard
cAMP concentration (pmol/50 μL), and R2 = 0.9368.
The cAMP content of the measured
sample was obtained from the standard curve. Finally, cAMP
concentrations in dormant and non-dormant chlamydospores of SDH035 and
DH055 isolates were obtained, and the results are shown in Figures 2A
and 2B. As shown in the figure, the content of cAMP in resting spores of
both strains was significantly higher than that in non-resting spores
(p <0.05).