1.1 Test strains
The D. flagrans isolates used in this study were SDH035 (CGMCC
KY288614.1) and DH055 (CGMCC: KY419119.1). The gene database entry
number is KU881774.1, which was isolated and purified from sheep feces
by using an improved spreading separation technique [2] (Wang et
al., 2015). The isolate was stored in a refrigerator at 4 ℃ on a test
tube containing 2% corn meal agar (Beijing Wanjia First chemical
Biotechnology Co., LTD.). The mycelium and spores were taken from the
preserved inclined plane, inoculated on a potato dextrose agar (PDA,
Beijing Landbridge Technology Co., LTD.) plate containing
chloramphenicol, and incubated at 28 ℃ for 7 days.
1.2 Production and purification of chlamydospores