Plant Extract Preparation
First, some plants were collected from the campus of Islamic Azad University, Science and Research Branch of Tehran and were identified by the Herbarium Center of Shahid Beheshti University with the herbarium code HSBU-2021982 for confirmation of botany and then dried in the shadow for 3 days and powdered. Maceration was used to extract this plant. 5g of p.avicule L. powder weighed by a sensitive scale and poured into an Autoclaved screw bottle and then 100 ml of mixed solvent (70 ml of Absolute ethanol + 30 ml of Distilled water) was prepared for the hydroalcoholic extract to be added to it and the solution was placed in the water bath with 80-90°C for 45minutes and the extract solution was filtered using Watman Filter Paper No. 2. After extraction, the extract was concentrated and the extract solvent was removed by rotary vacuum evaporation (Rv 10 Digital, Germany). and After using the rotary, to dry the extract at 25°C(room temperature), it was poured into a watch glass and placed in a suitable place to dry. The extract powder was then poured into a suitable container and kept at a suitable temperature until use.