Antibacterial assay
The agar disk diffusion method
was utilized to assess the antibacterial effectiveness of the silver
nanoparticles that were produced. This was done to confirm the
antibacterial properties of the synthesized silver nanoparticles.
Nutrient agar medium was made and sterilized, and 10 ml of culture
medium was added to each Petri dish. After freezing the culture medium,
the investigated bacteria were cultured in nutrient agar culture medium
on Petri plates. Then, different concentrations of synthesized silver
nanoparticles (5, 10, 20, 40 (%v/v)) were poured on 6 mm discs (Pad tan
Teb) sterilized in an autoclave and on the nutrient agar plates utilized
and then incubated at 37°C for a period of 24 hours. Following this, the
diameter of the growth inhibition zone is measured in millimeters
(39,41).