Synthesis of silver nanoparticles
For the synthesis of silver nanoparticles 10 ml of the extract 90ml of silver nitrate (AgNO3) (German company Merck) 2 mM was added and to reduce silver ions, the solution was kept at 25°C and kept in the dark overnight (23). The alteration of the solution’s hue from a light-yellow shade to a deep brown or black hue serves as evidence of the creation of silver nanoparticles (24). Subsequently, the solution comprising the nanoparticles underwent centrifugation at 8000 revolutions per minute for a duration of 20 minutes, following which the supernatant was decanted and the remaining sediment was dried. The obtained silver nanoparticles were stored inside the microtube at 4°C for further study (25).
Characterization of AgNPs
Ultraviolet-visible spectroscopy to identify AgNPs
To detect the presence of silver, UV-Vis spectrophotometry (PG instrument two-beam) was utilized to scan the absorption peak within the 350-700 nm range, as silver exhibits absorption within this range.