Antibacterial assay
The agar disk diffusion method was utilized to assess the antibacterial effectiveness of the silver nanoparticles that were produced. This was done to confirm the antibacterial properties of the synthesized silver nanoparticles. Nutrient agar medium was made and sterilized, and 10 ml of culture medium was added to each Petri dish. After freezing the culture medium, the investigated bacteria were cultured in nutrient agar culture medium on Petri plates. Then, different concentrations of synthesized silver nanoparticles (5, 10, 20, 40 (%v/v)) were poured on 6 mm discs (Pad tan Teb) sterilized in an autoclave and on the nutrient agar plates utilized and then incubated at 37°C for a period of 24 hours. Following this, the diameter of the growth inhibition zone is measured in millimeters (39,41).