Synthesis of silver nanoparticles
For the synthesis of silver nanoparticles 10 ml of the extract 90ml of silver nitrate (AgNO3) (German company
Merck) 2 mM was added and to reduce silver ions, the solution was
kept at 25°C and kept in the dark overnight (23). The alteration of the
solution’s hue from a light-yellow shade to a deep brown or black hue
serves as evidence of the creation of silver nanoparticles (24).
Subsequently, the solution comprising the nanoparticles underwent
centrifugation at 8000 revolutions per minute for a duration of 20
minutes, following which the supernatant was decanted and the remaining
sediment was dried. The obtained silver nanoparticles were stored inside
the microtube at 4°C for further study (25).
Characterization of
AgNPs
Ultraviolet-visible
spectroscopy to identify AgNPs
To detect the presence of silver, UV-Vis spectrophotometry (PG
instrument two-beam) was utilized to scan the absorption peak within the
350-700 nm range, as silver exhibits absorption within this range.