Plant Extract Preparation
First, some plants were collected from the campus of Islamic Azad
University, Science and Research Branch of Tehran and were identified by
the Herbarium Center of Shahid Beheshti University with the herbarium
code HSBU-2021982 for confirmation of botany and then dried in the
shadow for 3 days and powdered. Maceration was used to extract this
plant. 5g of p.avicule L. powder weighed by a sensitive scale and
poured into an Autoclaved screw bottle and then 100 ml of mixed
solvent (70 ml of Absolute ethanol + 30 ml of Distilled
water) was prepared for the hydroalcoholic extract to be added to it and
the solution was placed in the water bath with 80-90°C for 45minutes and the extract solution was filtered using Watman Filter
Paper No. 2. After extraction, the extract was concentrated and the
extract solvent was removed by rotary vacuum evaporation (Rv 10 Digital,
Germany). and After using the rotary, to dry the extract at 25°C(room temperature), it was poured into a watch glass and placed in a
suitable place to dry. The extract powder was then poured into a
suitable container and kept at a suitable temperature until use.