Transient electron microscope (TEM)
To determine the size and distribution dispersion of the synthesized
silver nanoparticles, the extract of polygonum aviculare L.plant, 912AB-LEO model electron microscope with an applied voltage of
120 kV was used to emit an electron beam.
Evaluation of antioxidant
properties by free radical scavenging (DPPH)
To evaluate the antioxidant activity of synthesized silver nanoparticles
the DPPH solution 0.004% was prepared. Then concentrations of
10, 50, 75, and 100 mg/Lof nanoparticles were treated with DPPH solution. After being kept in
the dark for half an hour, the absorbance of the sample was measured via
UV-vis spectroscopy at a wavelength of 517 nm. Ascorbic acid was
employed as a standard antioxidant reference purpose. The scavenging
activity of free radicals was determined using the following formula:
%RSC = \(\frac{Ab-As}{\text{Ab}}\) ×100
Ab= DPPH Absorbance, As= Sample or
Standard Absorbance
The antioxidant activity of each sample was expressed in terms of
IC50 (concentration required to inhibit the 50% DPPH
radical formation) calculated from the inhibition curve.