Transient electron microscope (TEM)
To determine the size and distribution dispersion of the synthesized silver nanoparticles, the extract of polygonum aviculare L.plant, 912AB-LEO model electron microscope with an applied voltage of 120 kV was used to emit an electron beam.
Evaluation of antioxidant properties by free radical scavenging (DPPH)
To evaluate the antioxidant activity of synthesized silver nanoparticles the DPPH solution 0.004% was prepared. Then concentrations of 10, 50, 75, and 100 mg/Lof nanoparticles were treated with DPPH solution. After being kept in the dark for half an hour, the absorbance of the sample was measured via UV-vis spectroscopy at a wavelength of 517 nm. Ascorbic acid was employed as a standard antioxidant reference purpose. The scavenging activity of free radicals was determined using the following formula: %RSC = \(\frac{Ab-As}{\text{Ab}}\) ×100
Ab= DPPH Absorbance, As= Sample or Standard Absorbance
The antioxidant activity of each sample was expressed in terms of IC50 (concentration required to inhibit the 50% DPPH radical formation) calculated from the inhibition curve.