Additional files
Additional file 1: Figure S1. CRISPR/Cpf1 mediated homologous
recombination. C. glutamicum was transformed with the
pJYS3_Amp_DT_[Lp-Knr-Lt] vector. The vector was
incorporated into the genomic DNA after the first cross-linking in the
promoter region of the ldhA gene. The vector accessories and theldhA gene are arranged in tandem. The selection marker gene
(Knr) on the vector part (A) and ldhA gene (B)
was concomitantly detected.
Additional file 2: Table S1. Primers design to construct
CRISPR-cpf1 gene editing vector.
Table 1. Hydrolysis rate (%) of softwoods and hardwoods depending on
pretreatment methods.