Analysis of cell wall hydrophobicity
The cell wall hydrophobicity of the cultivated cells was determined
using a modified microbial adhesion to hydrocarbon (MATH) assay
(Kahar et al., 2022;
Van Mulders et al., 2009). After deflocculation with 50 mM
ethylenediaminetetraacetic acid (EDTA, pH 7) and resuspension in 2 mL of
0.9% sodium chloride solution, the samples were measured at 600 nm
using a spectrophotometer (UVmini-1240, Shimadzu, Japan) to obtainA0 . Subsequently, 0.4 mL of octane was added to
the solutions, followed by vortexing at maximum speed for 60 sec. After
allowing the mixture to stand at room temperature for 10 min, the water
phase containing nonhydrophobic cells was separated and measured at 600
nm to obtain A1 . The cell wall hydrophobicity was
then calculated using the following formula:
\begin{equation}
Cell\ wall\ hydrophobicity\ =\ \left(1-\frac{A_{1}}{A_{0}}\right)\cdot 100\nonumber \\
\end{equation}