2.7 Analysis of cell viability
Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) (Beyotime, Shanghai, China), following the manufacturer’s instructions. HT22 cells were seeded in 96-well plates under normoxic conditions until cell adherence. The cells were subjected to oxygen-glucose deprivation (OGD) for a defined period of time, followed by re-establishment of normal culture conditions for an additional 24 hours with or without therapeutic intervention. The optical density (OD) value at 450 nm in each well was quantified using a microplate reader (Bio-Rad, Hercules, California, USA).