2.13 Enzyme‑linked immunosorbent assay (ELISA)
The brain tissues were thoroughly ground and the supernatant of the
homogenate was collected after centrifugation. The cell culture
supernatants were also collected. The IL-1β, IL-6, and TNF-α levels were
detected using a commercial ELISA kit (Nanjing Jiancheng Bioengineering
Institute, China).