2.7 Analysis of cell viability
Cell viability was assessed using the Cell Counting Kit-8 (CCK-8)
(Beyotime, Shanghai, China), following the manufacturer’s instructions.
HT22 cells were seeded in 96-well plates under normoxic conditions until
cell adherence. The cells were subjected to oxygen-glucose deprivation
(OGD) for a defined period of time, followed by re-establishment of
normal culture conditions for an additional 24 hours with or without
therapeutic intervention. The optical density (OD) value at 450 nm in
each well was quantified using a microplate reader (Bio-Rad, Hercules,
California, USA).