2.3 Neurological score evaluation
The mice that expired shortly after MCAO/R were excluded. Evaluations
were conducted 24 hours post-reperfusion, immediately prior to brain
tissue sampling. The Modified Neurological Severity Score
(mNSS)36,37 was employed to assess the severity of
nerve injury models in all experimental animals. The degree of
neurological deficits was graded on a scale from 0 to 18, with higher
scores indicating greater damage.