Assessment of working memory
Working memory was assessed by the novel object recognition test (Lueptow, 2017). Mice were acclimated for 2 d by placement in the empty testing box for 10 min. On the day of testing, the mouse was placed in the same box with two identical objects for 10 min. One h later, the mouse was placed back in the same box for 5 min with one of the familiar objects replaced by a novel object. The mouse’s interactions with the objects (nose point entering a zone 2 cm wide around the object) were tracked using automated software (Ethovision XT, Noldus Information Technology BV, Wageningen, The Netherlands).