SARS-CoV-2 neutralization assay
As previously described(22), for the virus neutralization assay, sera
were incubated for 30 min at 56°C in order to inactivate complement
factors. Single cycle VSV∗ΔG(FLuc) pseudoviruses bearing the SARS-CoV-2
WT S (D614G) protein in the envelope were incubated with quadruplicates
of two-fold serial dilutions of immune sera in 96-well plates prior to
infection of Vero E6 cells (1x104 cells / well) in DMEM + 10% FBS (Life
Technologies). At 18 hours post infection, firefly luciferase (FLuc)
reporter activity was determined as previously described S5 using a
CentroXS LB960 (Berthold) and the reciprocal antibody dilution causing
50% inhibition of the luciferase reporter was calculated (PVND50).