Fig.12(A)Representative images of wound tissue sections stained by Masson in different treatment groups on day 3 (Scale bar ¼ 1 mm and 50 μm). (B) Quantification of the collagen content around laser effect zone in different treatment groups on day 3 (n ¼ 3). (C) Quantification of the collagen fractional area around laser effect zone in different treatment groups on day 3 (n ¼ 3).
As shown in Fig.13(A), the wound direction of the four groups of samples on the 3rd day of healing and the distribution of different types of collagens around the wound were separately labeled. It can be observed that the proportion of two types of collagens in the 90° laser group was the most prominent, up to 2.68, and the COL-1 fiber content was significantly higher than that in the other three groups (as shown in Fig.13(C)). This indicated that the tissue of the samples in this group was relatively stiff after the first laser welding, but the strength was higher, which met the standard of good healing. According to Fig.13(B), the ratio of COL-1 fibers and COL-3 fibers of samples under 90turned was also the highest. However, the ratio of COL-1 and COL-3 fibers in the samples in two laser groups with incident angles were significantly lower than that in the control group, indicating that although the collagen proliferation of the samples in these two groups was higher than that in the control group without any operation, while COL-3 fiber was the collagen that prolifically proliferated in 60 laser and 30 laser groups, the tissue was softer, and the elasticity was significantly higher than that of the 90° laser group at the 3rd day. After the first laser welding, the higher elasticity of wound tissue indicated that the samples were not prone to scar, and the thermal damage caused by laser was also relatively low under 60 laser and 30 laser.