Fig.7 Pathological examination of wound tissue (A) Representative images of evolution of microstructure during healing processes by Sirius Red staining (\(\times 400\)). (B) Quantitative data of Content of COL-1 fiber after the two stages of laser welding at different time points (n ¼ 3). Data represent mean \(\pm\) SD; *, P < 0.05, **, P < 0.01.
Overall cell proliferation during wound healing in rats
Immunofluorescence staining was utilized to perform immune expression of Fibronectin antibodies. As depicted in Fig.8(A), it showcases the progressive evolutions in fibroblast expression throughout the entire healing process—from day 3 up until day 14—following two laser welding sessions. On the third day after the initial laser welding, it was noted that fibroblast proliferation was most prominent in the 60° laser group, reaching a fluorescence intensity of 1.28 (as demonstrated in Fig.8(B)) after normalization, with substantial proliferation observed within the dermis. Post the second laser welding, the fluorescence expressions within the groups stabilized around a value of 1.25, and noteworthy fibroblast clustering was detected in the 60° laser group. By day 14, the fibroblast content within the laser groups returned to normal levels, with the primary fluorescence expression concentrated within the middle and lower regions of the dermis [53-58]. The fibroblast expression in the 30° laser group was still densely concentrated, indicating a slower healing process compared to the other two groups.