2.1 Specimen collection and library preparation
Species sequenced in this study included five species within themacrotis group and one closely related species R. pusillus(Table 1). Samples of the brain, liver, and cochlear were biopsied
quickly after euthanasia, placed in RNA later, and then stored at -80℃
until RNA extraction. To avoid the influence of sex, we chose mature
females and ensure none were pregnant. Total RNA from these tissues was
extracted using TRIzol reagent (Invitrogen Corp., Carlsbad, CA). RNA
purification was performed using an RNeasy Mini Kit (Qiagen, Chatsworth,
CA). Library constructions from the brain, liver, and cochlear of each
species according to the Illumina Hiseq 2500 RNA sample preparation kit
(Illumina, San Diego, CA). We constructed two paired-end libraries with
insert sizes of 150 base pairs (bp). All these original data have been
deposited into the NCBI Sequence Read Archive database (Accession
Number: SAMN35883750-SAMN35883755) (Supporting Information Table S1).