Bacteria Quantification method No. of acetylation sites No. of acetylation proteins Acetylome changes Reference
Bacillus nematocida
TMT labeling/ Immunoaffinity enrichment with LC−MS/MS
129
349
During pathogenesis, the acetylation levels of 18 lysine sites were upregulated and 19 lysine sites in were downregulated
Sun et al., 2018
Streptococcus mutans (Bacterial biofilm growth (SMB) vs planktonic growth (SMP))
973
445
In SMB glucosyltransferase-SI, glucosyltransferase-I, and glucosyltransferase-S were downregulated
Lei et al., 2021
C. acetobutylicum Stable isotope dimethyl labeling/ Immunoaffinity enrichment with nano-HPLC/mass spectrometric analysis 458 254 No differences were observed in the overall acetylation levels of whole cell lysates during the exponential and stationary phases. During the transitional phase, slight acetylation was observed Xu et al., 2018
Pseudomonas aeruginosa
Label-free/2D immunoaffinity approach coupled to nano HPLC/MS/MS
1102
522
The carbon source does not produce significant changes in the number of acetylated peptides.
Gaviard et al., 2018
Escherichia coli
Label-free/ Immunoaffinity enrichment with LC−MS/MS
2730
806
For cells grown in the presence of glucose the number of upregulated acetyl lysine sites is substantially larger in the ackA mutant than in the cobB mutant (>8-fold for acetyl sites, >5-fold for proteins) Acetyl lysine sites decreased slightly in the yfiQ mutant. Acetylation is both non-enzymatic and specific
Kun et al., 2014
Bacillus subtilis
2372
841
In the stationary phase 271 acetylation sites were significantly downregulated and 92 were upregulated. In the transition from the log to the stat phase, DNA-mediated transformation proteins were upregulated, and proteins involved in translation were downregulated
Carabetta et al., 2016
Escherichia coli 818 434 KATs overexpression significantly increased the number of acetylated proteins and lysine residues. Christensen et al., 2018