2.3 Detection of hematological parameters
2.3.1 Detection of White blood cells
2 ml of venous blood of the subjects was drawn into a vacuum blood collection tube containing EDTA-K2 anticoagulant. Blood cells were automatically absorbed, diluted, stained, and measured by routine analyzer (XN-1000, SYSMEX) to obtain the information of blood cells, and then the WBC, L%, and LYM were recorded.
2.3.2 Detection of Liver and kidney function
The venous blood was drawn in a vacuum blood collection tube without anticoagulant. After standing at room temperature for 30 min, the blood was centrifuged at 3500 rpm for 5 min to obtain serum. If the specimen can’t be detected in time, refrigerate the centrifuged specimen in a refrigerator at 2-8℃. The liver and renal function were detected in Automatic biochemical analyzer (COBASC 701, Roche), and then ALT, AST, GGT, UREA, and CREA were recorded.