Application of Shear Forces
This study uses the cone and plate bioreactors (CPB) to investigate the
fluid shear stress experienced by endothelial cells (EECs) in the left
ventricular outflow tract (LVOT) of patients with DSS. The CPB system
operates based on the following principle: an inverted cone is
positioned above a stationary flat plate, creating a narrow gap through
which fluid flows. This arrangement ensures the distribution of uniform
shear stress across the surface [46].
To control the fluid flow, a magnetic stir plate is employed, which
enables adjustment of the cone’s angular velocity by modifying the
revolutions per minute (RPM) setting on the control unit. This mechanism
allows for precise manipulation of the shear stress experienced by the
EECs in the CPB system [46].