FIGURES LEGENDS
Fig. 1. Phases of the extracts of different polarities (hexane,
ethyl acetate, methanol) using the mycelial mass and broth of the eight
isolates that produce the most intense colorants (Penicillium sp.
P3SO332, Clonostachys sp. P2SO329, Penicillium sp.P3SO224, Penicillium sp. P3SO322, Phoma sp. P2SO127,Penicillium sp. P3SO133, Penicillium sp. P2SO334,Monascus sp. C02172R).
Fig. 2. The evolutionary history was inferred by using the
maximum-Likelihood likelihood method and the Tamura-Nei (1993) model.
The percentage of trees in which the associated taxa clustered together
in the bootstrap test (1,000 replicates) is shown next to the branches.
The tree is drawn to scale, with branch lengths measured in the number
of substitutions per site. This analysis involved 12 nucleotide
sequences. The green dots indicate the isolates from this study and the
others are ITS sequences of colorant-producing fungi. There was a total
of 738 positions in the final dataset. Evolutionary analyses were
conducted in MEGA X v10.2.4.
Fig. 3. Effect of different carbon and nitrogen sources in the
production of colorants with the three selected fungi.Penicillium gravinicasei P3SO332 (A, B);Clonostachys rosea P2SO329 (C, D); Penicilliumsclerotiorum P3SO224 (E, F). Different letters represent
significant differences according to the Student’s t-test
(p< 0. 05).
Fig. 4. Structure of sclerotiorin present in the EtOAc extract
of the fungi P . sclerotiorum P3SO224 (1) and penicillic
acid (2) present in the EtOAc extract of Clonostachys roseaP2SO329. *Structures designed using Chemdraw 19.0
Fig. 5. Polyester, silk and cotton fabrics dyed with crude
colorants extracted from Clonostachys rosea P2SO329 (CR9),Penicillium gravinicasei P3SO332 (PG2) and Penicillium
sclerotiorum P3SO224 (PS4).
Fig. 6. Color and aspect of cream formulations comprising the
colorant extracts (0.05 mg per gram of the formulation) produced byPenicillium sclerotiorum P3SO224 (PS4 10 mg), Clonostachys
rosea P2SO329 (CR9 14 mg), Penicillium gravinicasei P3SO332 (PG2
14 mg) and commercial colorant (yolk-yellow liquid food coloring - MIX).
Fig. 7. Soap formulation using colorant 1 (0.05 mg per gram)
obtained from Penicillium sclerotiorum P3SO224. Commercial
colorant (yolk-yellow liquid food coloring - MIX).