2.1 Collection of samples and isolation of fungi
Superficial soil and litter were collected in September 2018 in a forest
zone located in the Campina Biological Reserve (2°35’19.19” S,
60°1’59.91” W) Manaus, Brazil. The samples were collected at the
following three points: point 1 (-2.5891030, -60.0319530), point 2
(-2.589249, -60.031893) and point 3 (-2.589368, -60.031712).
Approximately 5 g of superficial soil and litter were collected with
clean, dry and sterile polythene bags and a sterile spatula. From the
collected samples, 1 g was added to 9 mL of sterile distilled water and
serial dilution was performed to dilute the sample. The suspension (0.1
mL) was added to sterile Petri dishes in duplicates containing sterile
Sabouraud agar and the spread plate technique was used. Plates were
incubated at 28 ºC±2 for 5-7 days [18].