2. 1 P. berghei Scd localized primarily in the
endoplasmic reticulum
To study the expression and localization of Scd in the P. bergheilife cycle, the gene was endogenously tagged with 3XHA by using double
crossover homologous recombination (Fig. S1A). The correct integration
of the targeting cassette at the Scd locus was confirmed by
diagnostic PCR (Fig. S1B). Furthermore, the expression of Scd-3XHA was
confirmed by western blotting using an anti-HA antibody (Fig. S1C).
Transgenic parasites were analyzed throughout the parasite life cycle
stages, and we found that the C-terminal tag did not affect parasite
development (Fig. S1D and E). Previous studies have demonstrated the
association of SCD with the endoplasmic reticulum (Gratraud et
al. , 2009). By using Pf Scd-GFP transgenic parasites, it was
shown that P. falciparum SCD localized to the ER in asexual blood
stages. Similarly, we performed colocalization studies with the ER
marker Bip and found that P. berghei was expressed in blood and
liver stages and primarily colocalized to the ER (Fig. 1A and B).