3.3.4 Comparative and analysis of morphological and molecular
identification results of AM fungi
The result of morphological identification showed that there were 9
genus and 20 species of AM fungi after wet sieving, while
high-throughput sequencing amplified and sequenced the DNA extracted
from the mixed soil from each small sample, and 4 genera and 11 virtual
species were identified. The genus Diversispora detected by
high-throughput sequencing means was not found in the morphological
identification, while morphological identification revealed six genera
which were not identified by high-throughput. The change trend of AM
fungal diversity calculated by the two methods was the same, and the AM
fungal diversity index used in the subsequent analysis was the result of
morphological identification.