3.3.4 Comparative and analysis of morphological and molecular identification results of AM fungi
The result of morphological identification showed that there were 9 genus and 20 species of AM fungi after wet sieving, while high-throughput sequencing amplified and sequenced the DNA extracted from the mixed soil from each small sample, and 4 genera and 11 virtual species were identified. The genus Diversispora detected by high-throughput sequencing means was not found in the morphological identification, while morphological identification revealed six genera which were not identified by high-throughput. The change trend of AM fungal diversity calculated by the two methods was the same, and the AM fungal diversity index used in the subsequent analysis was the result of morphological identification.