Figure.4. The expression trend of 11 vision-related functional genes ofO. oratoria compound eyes in five lighting scenarios based on transcriptome and qRT-PCR datasets.
Meanwhile, few vision-related functional proteins were identified, including arrestin protein, sodium-coupled neutral amino acid transporter protein, sodium/potassium/calcium exchanger protein, transient receptor potential channel protein, retinal dehydrogenase protein, and G protein-coupled receptor protein. Among them, arrestin protein and sodium-coupled neutral amino acid transporter protein were inferred to contribute to the LCPL-recognition of O. oratoria compound eye. The expression trends of five proteins based on proteomic sequencing and PRM were consistent. Similar to gene expression levels, the expression levels of vision-related proteins in O. oratoria compound eyes exposed to NL or LCPL scenarios showed the higher values (Figure.5).