2.1 Ethics
O. oratoria is not an endangered or protected animal in any
country, and no special ethical approval are required. All O.
oratoria s used in subsequent experiments were placed on ice for frozen
anesthesia before dissection to minimize the suffering. Meanwhile, we
rigorously conducted the experiments under the guidance of the
National Institutes of Health
Guidelines for the Care and Use of Laboratory Animals (NIH Publication
No. 8023, revised in 1978) and Institutional Animal Care and Use
Committee of Yantai University.