5. Conclusion
A chromosomal-level genome of C. japonica has been published for the first time by integrating the short Illumina reads, long PacBio reads, and Hi-C reads. This is a highly assembled and reliable genome and it enriches the crustacean genome database. Meanwhile, we successfully annotated the repeat elements, coding genes, miRNAs, tRNA, rRNAs, and snRNAs in the C. japonica genome. These information will be necessary for future research into C. japonica biology. Additionally, the whole-genome resequencing data of C. japonicawith different gender provided a genome-wide scan of SNPs and InDels, which were used to predict the sex-determining chromosome of C. japonica . C. japonica is a crustacean with strong desiccation tolerance. This study used comparative genome analysis to study the evolutionary history of C. japonica , as well as a series of genes that may contribute to desiccation adaption in C. japonica . The present results were beneficial in elucidating the evolution process inC. japonica and other marine organisms under desiccation stress.