3.5 Sex-determining region identification of C. japonica
The whole-genome resequencing of 20 C. japonica individuals from different genders provided a genome-wide scan of SNPs and InDels. Results showed 9 SNP variants (Number: 1,811,614; Table 6) and 12 InDel variants (Number: 1,671,995; Table 7). The allele in chromosome 37 ofC. japonica had the highest F STaggregation, which means that chromosome 37 is the sex-determining chromosome of C. japonica (Figure 3). Furthermore, we calculated the genotype frequencies using bulk segregant analysis (BSA), and the distribution pattern of Euclidean distance (ED) showed that a difference between 0 and 10,120,000 bp in the chromosome 37 of female and maleC. japonica (Figure 4).