5. Conclusion
A chromosomal-level genome of C. japonica has been published for
the first time by integrating the short Illumina reads, long PacBio
reads, and Hi-C reads. This is a highly assembled and reliable genome
and it enriches the crustacean genome database. Meanwhile, we
successfully annotated the repeat elements, coding genes, miRNAs, tRNA,
rRNAs, and snRNAs in the C. japonica genome. These information
will be necessary for future research into C. japonica biology.
Additionally, the whole-genome resequencing data of C. japonicawith different gender provided a genome-wide scan of SNPs and InDels,
which were used to predict the sex-determining chromosome of C.
japonica . C. japonica is a crustacean with strong desiccation
tolerance. This study used comparative genome analysis to study the
evolutionary history of C. japonica , as well as a series of genes
that may contribute to desiccation adaption in C. japonica . The
present results were beneficial in elucidating the evolution process inC. japonica and other marine organisms under desiccation stress.