4.2 More accurate evolutionary history of C. japonica
The presented genomic resource can help us better understand the
evolutionary history of C. japonica . The differentiation of
conserved single-copy orthologous genes always leads to species
differentiation; thus, we believe that the evolutionary history ofC. japonica can be more accurately deetermined using widespread
single-copy orthologous genes (Liang et al., 2013; Fitch, 1970). In the
present study, 968 single-copy orthologous genes were obtained and then
applied to construct the phylogenetic relationship of C.
japonica . Expectedly, the constructed phylogenetic structure is
considerably congruent with the prevailing morphological and molecular
biological views that crabs (P. trituberculatus and C.
japonica ) from family Portunidae eventually cluster into one branch.
Moreover, the evolutionary sequence of C. japonica was identified
based on differentiation times, and these results provided evidence to
prove the existence of “carcinization” (Keiler et al., 2017; Cui et
al., 2021). In fact, 1,138 unique gene families were found in the
genome, and these gene families can contribute to C.
japonica -specific adaptation (Wissler et al., 2013). Additionally, the
relatively evolution rates of different species may also mean
differences in environmental stability (Tang et al., 2020).