3.1 Evaluation of C. japonica genomic characteristics
A total of 164.57 Gb of Illumina data, which correspond to 1,101,954,864 Illumina reads, were generated from the Illumina NovaSeq-6000 platform. The Q20, Q30, and GC content of the Illumina reads were 96.70%, 91.57%, and 42.29%, respectively. After 17-mer was set, 123,219,056,032 K-mers with a depth of 84 were obtained. TheC. japonica genome size assessed by K-mer analysis was 1,398 Mb. After the negative effect caused by incorrect K-mer was eliminated, the revisedC. japonica genome size was 1,384 Mb. Meanwhile, the heterozygous ratio and repeat of Illumina reads were 1.09% and 61.24%, respectively.