Treatments
Five isonitrogenous and isolipidic feeds were prepared containing increasing concentrations of cholesterol. Treatments were (T1): 0% cholesterol, (T2): 0.5% cholesterol, (T3): 1% cholesterol, (T4): 1.5% cholesterol and (T5): 2% cholesterol (Table 1). Ingredients were mixed with hot water and extruded in a meat grinder through a 2mm die then dried in a forced-air oven at 45°C until constant weight.