Animals
This study was conducted on 20 healthy horses with different breeds
including Koninklijk Warmbloed Paardenstamboek Nederland (KWPN),
crossbreed, thoroughbred, and Friesian from different breeding centers
located in Tehran province. Horses under study had neither clinical
signs nor a history of eye disorders. No evidence of ocular inflammation
or infection was observed during ophthalmic examinations conducted by a
veterinarian. Examinations consisted of menace test and assessment of
both eyeballs’ location, size of both eyes, motility of both eyes, and
the existence of any ocular discharge. No history of antimicrobials or
antifungal drugs administration in 2 weeks before specimen collection
was also confirmed. Horses were divided into three different age
intervals including 1-5 years old, 5-10 years old, and older than 10
years old. Characteristics of animals are shown in Table 1.