Antibiotic sensitivity test
After the identification of bacteria, cultured medium plates were used
for antibiogram tests. Antibiotic discs of 10 effective antibiotics for
horses highly utilized in similar studies, including Colistin,
ceftiofur, florfenicol, amoxicillin, ampicillin, enrofloxacin,
ciprofloxacin, trimethoprim-sulfamethoxazole, neomycin, and gentamycin
were placed on the media using forceps. Plates’ incubation was done at
37◦C for 24-48 hours. Zones of growth inhibition were
measured and recorded as sensitive or intermediate(Tahoun et al., 2022,
Baran et al., 2015).