Antibiotic sensitivity test
After the identification of bacteria, cultured medium plates were used for antibiogram tests. Antibiotic discs of 10 effective antibiotics for horses highly utilized in similar studies, including Colistin, ceftiofur, florfenicol, amoxicillin, ampicillin, enrofloxacin, ciprofloxacin, trimethoprim-sulfamethoxazole, neomycin, and gentamycin were placed on the media using forceps. Plates’ incubation was done at 37C for 24-48 hours. Zones of growth inhibition were measured and recorded as sensitive or intermediate(Tahoun et al., 2022, Baran et al., 2015).