Figure 5. Different Pik-H4 expressions facing the rice blast fungus or effector
(A ) Punch inoculation in different plants overexpressingPik1-H4 or Pik2-H4 . Bar=1 cm. (B and C ) Measurement of lesion length and sporulation rate from (A ). Data represent means ± standard deviation, One asterisk indicates P <0.05, two asterisks indicate P <0.01 (One-way ANOVA with student’st -test, n=5). (D and E ) The relative expression levels measured by qRT-PCR of Pik genes from uninfected plants in (A ). Data represent means ± standard deviation, One asterisk indicates P <0.05, two asterisks indicateP <0.01 (One-way ANOVA with student’s t -test, n=3). (F ) Relative fluorescence intensity compared to theRFP ::PPik-H4 ::GFP (P) plasmid in tobacco transient assays, n=10. 1, P35S ::Pik1-H4 ::Flag . 2, P35S ::Pik2-H4 ::Ha . A, P35S ::AvrPik-E ::Myb . The dashed line represents intensity value=1. (G ) Ion leakage assay measuring Pik-H4 -mediated cell death in tobacco at 3 dpi. The Agrobacteria expressing Pik1-H4-Flag and Pik2-H4-HA were mixed in different volumes with or without AvrPik-E-Myc. Two asterisks indicate P <0.01 (One-way ANOVA with student’s t -test, n=5).