Figure 6. Subcellular location of Pik1-H4 and Pik2-H4 in leaf sheaths in planta
(A - D ) Overexpression ofPik1-H4-GFP in LTH. (A ) Pik1-H4-GFP was in the membrane and cytosol. (B ) Fluorescence intensity of Pik1-H4-GFP and membrane dye FM4-64 along the dotted line in (A ). (C ) Pik1-H4-GFP was in the nucleus and vesicles. (D ) Pik1-H4-GFP was in vesicles when infected. (E - H ) Overexpression ofPik2-H4-GFP in LTH. (E ) Pik2-H4-GFP was in the membrane and cytosol. (F ) Fluorescence intensity of Pik2-H4-GFP and membrane dye FM4-64 along the dotted line in (E ). (G ) Pik2-H4-GFP was in nucleus. (H ) Pik2-H4-GFP was in the nucleus when infected. (I and J ) Overexpression ofPik1-H4-GFP in Pik-H4 NILs. (I ) Pik1-H4-GFP was in the membrane, cytosol, nucleus and vesicles. (K and L ) Overexpression ofPik2-H4-GFP in Pik-H4 NILs. (K ) Pik2-H4-GFP was in the membrane, cytosol and nucleus. (J and L ) Pik1-H4-GFP and Pik2-H4-GFP signals were weakened when infected withM. oryzae in Pik-H4 NILs. FM4-64 was marked in red in (A and E ). In (C , D , G ,H , I - L ), chlorophyll A was marked in red and the nucleus was dyed with DAPI (blue). All the bars represent 10 μm.