Figure 6. Subcellular location of Pik1-H4 and
Pik2-H4 in leaf sheaths in planta
(A - D ) Overexpression ofPik1-H4-GFP in LTH. (A )
Pik1-H4-GFP was in the membrane and cytosol.
(B ) Fluorescence intensity of Pik1-H4-GFP and
membrane dye FM4-64 along the dotted line in (A ). (C )
Pik1-H4-GFP was in the nucleus and vesicles.
(D ) Pik1-H4-GFP was in vesicles when infected.
(E - H ) Overexpression ofPik2-H4-GFP in LTH. (E )
Pik2-H4-GFP was in the membrane and cytosol.
(F ) Fluorescence intensity of Pik2-H4-GFP and
membrane dye FM4-64 along the dotted line in (E ). (G )
Pik2-H4-GFP was in nucleus. (H )
Pik2-H4-GFP was in the nucleus when infected.
(I and J ) Overexpression ofPik1-H4-GFP in Pik-H4 NILs. (I )
Pik1-H4-GFP was in the membrane, cytosol, nucleus and
vesicles. (K and L ) Overexpression ofPik2-H4-GFP in Pik-H4 NILs. (K )
Pik2-H4-GFP was in the membrane, cytosol and nucleus.
(J and L ) Pik1-H4-GFP and
Pik2-H4-GFP signals were weakened when infected withM. oryzae in Pik-H4 NILs. FM4-64 was marked in red in
(A and E ). In (C , D , G ,H , I - L ), chlorophyll A was marked in red
and the nucleus was dyed with DAPI (blue). All the bars represent 10 μm.