Figure 3. Pik-H4 co-expressed in tissues and cells
(A ) The fluorescence microscopy ofRFP ::PPik-H4 ::GFP /Nip plants. The RFP and GFP signals represent the promoter activity ofPik1-H4 and Pik2-H4respectively. The letter “m” represents mesophyll tissue, and “vb” represents vascular bundles. (B , E and I ) Measurement of fluorescence intensity. (B and E ) were measured from (A ), (I ) was measured from (F ). One-way ANOVA with student’s t -test, n=30. (C ,G and H ) The linear regression of RFP and GFP signals. The F-test was employed to compare statistical models applied to a dataset through linear regression. (C ) was built from (A ), n=180. (G and H ) were constructed from (F ), n=30. (D ) Relative expression levels ofPik1-H4 and Pik2-H4. Data represent means ± standard deviation (One-way ANOVA with student’st -test, n=3). (F ) The confocal fluorescence microscopy. The letter “mc” represents mesophyll cell, and “vc” represents vascular cell.