Figure 2. Promoter analysis of different PPik-H4sequences
(A - C ) Minimal promoter region of
PPik-H4 . The schematic of a combination of two
directions is shown in (B ). (D - F ) Mutation
of bidirectional TATA boxes. The schematic is shown in (B ).
Data represent means ± standard deviation (n=10). The RFP andGFP relative fluorescence intensity compared to the native
promoter was shown in (G ), (I ), (G ) and
(I ). The lowercase letters (a, b, c) indicate the data were atP <0.01 level according to one-way ANOVA with Dunnett’s
test. The dashed lines represent the value of the emptyRFP ::GFP plasmid.