Figure 4. PPik-H4 activity was up-regulated after infection in RFP ::PPik-H4 ::GFP /Nip plants
(A and B ) The promoter activity upon infection in leaf tissue. The fluorescence intensity in (B ) was measured along the dotted line from (A ). The red and green dashed lines represent the average RFP and GFP intensity in a normal state in leaves. The grey line was measured in the bright field and indicated the lesion area when the values were lower. (C - E ) The promoter activity upon infection in the leaf vascular bundle. (D andE ) The relative fluorescence intensity compared to the mock treatment from (C ). Data represent means ± standard deviation (One-way ANOVA with student’s t -test, n=10). One asterisk indicates P <0.05, two asterisks indicateP <0.01. I, infected area. P, peripheral area of the infected area. E, early stage of infection. B, biotrophic stage. (F - K ) The confocal fluorescence microscopy in leaf sheaths. (F , G and J ), mesophyll cell. (H , I and K ), vascular bundles. (Gand I ), One-way ANOVA with student’s t -test, n=10. (J and K ) The linear regression from (F andH ), The F-test was employed to compare statistical models applied to a dataset through linear regression, n=10.