Figure 5. Different Pik-H4 expressions facing the rice blast
fungus or effector
(A ) Punch inoculation in different plants overexpressingPik1-H4 or Pik2-H4 . Bar=1
cm. (B and C ) Measurement of lesion length and
sporulation rate from (A ). Data represent means ± standard
deviation, One asterisk indicates P <0.05, two asterisks
indicate P <0.01 (One-way ANOVA with student’st -test, n=5). (D and E ) The relative expression
levels measured by qRT-PCR of Pik genes from uninfected plants in
(A ). Data represent means ± standard deviation, One asterisk
indicates P <0.05, two asterisks indicateP <0.01 (One-way ANOVA with student’s t -test,
n=3). (F ) Relative fluorescence intensity compared to theRFP ::PPik-H4 ::GFP (P) plasmid in
tobacco transient assays, n=10. 1,
P35S ::Pik1-H4 ::Flag .
2,
P35S ::Pik2-H4 ::Ha .
A, P35S ::AvrPik-E ::Myb . The dashed
line represents intensity value=1. (G ) Ion leakage assay
measuring Pik-H4 -mediated cell death in tobacco at 3 dpi. The
Agrobacteria expressing Pik1-H4-Flag and
Pik2-H4-HA were mixed in different volumes with or
without AvrPik-E-Myc. Two asterisks indicate P <0.01
(One-way ANOVA with student’s t -test, n=5).